The Department of Transportation (DOT) mandates drug and alcohol testing for employees in safety-sensitive transportation roles, including commercial drivers, pilots, and pipeline workers. This regulatory framework ensures public safety by standardizing the testing process across various modes of transit. Understanding how these results are processed and verified is necessary for both the individual being tested and the organization employing them. Knowing the proper channels for checking these results helps maintain adherence to regulations.
The Key Players in the DOT Results Chain
The process of generating a final DOT drug test result involves a precise chain of custody managed by several specialized entities. The initial step is handled by the Collector, who observes the specimen collection and ensures proper documentation on the Federal Drug Testing Custody and Control Form (CCF). The specimen is then sealed and sent to a Substance Abuse and Mental Health Services Administration (SAMHSA) certified Laboratory for screening.
Once the laboratory completes its analysis, the results are transmitted to the Medical Review Officer (MRO). The MRO is a licensed physician who reviews all positive and non-negative results to ensure scientific validity. This medical professional acts as an independent gatekeeper between the lab and the employer.
The Designated Employer Representative (DER) serves as the primary contact point for the employer regarding the testing program. The DER is the only person authorized to receive the final, verified test result from the MRO and take appropriate employment action.
How the Medical Review Officer Finalizes Results
The MRO’s involvement represents the medical and scientific verification stage necessary before any official outcome is declared. When the laboratory reports a negative result, the MRO confirms the documentation is complete and immediately releases the finding to the Designated Employer Representative. This streamlined process allows for quick notification when no prohibited substances are detected.
If the laboratory reports a non-negative result, the MRO must initiate a confidential interview with the tested employee. During this consultation, the MRO seeks to determine if there is an alternative medical explanation for the presence of the substance, such as a legally valid prescription. The employee must provide documentation proving the legal use of a medication that could cause the non-negative finding.
The MRO makes the final determination based on the scientific evidence and the medical information gathered. If a legitimate medical explanation is provided and verified, the MRO reports the result as negative. If no valid explanation exists, the MRO reports the result as a verified positive.
Checking Your Results as a Tested Employee
An employee’s method for checking a DOT result differs significantly based on the outcome. If the specimen yields a negative result, the employee generally receives confirmation from the employer or simply returns to work. The absence of communication from the MRO is often the clearest indication that the result was negative and the process is complete.
If the result is non-negative, the MRO is federally obligated to contact the employee directly via telephone. This contact typically occurs 24 to 72 hours after the MRO receives the lab report. The employee should ensure their contact information provided to the collector is accurate to avoid missing this outreach.
If an employee has not heard from the MRO and has not been cleared to return to work after the standard timeline, they have the right to inquire about the status. The employee should direct this request to the Designated Employer Representative (DER). The DER holds the official record and communicates the final disposition to the employee.
Checking and Documenting Results as an Employer
The Designated Employer Representative (DER) acts as the final recipient of the verified drug test results from the MRO. The MRO must transmit the official report using secure methods, such as a secure fax line, encrypted email, or a dedicated electronic reporting system. This security protocol protects the sensitive personal health information of the tested individual.
Upon receiving the MRO report, the DER must check that the document is complete and contains all required information, including the MRO’s signature and the final determination. This verification step ensures adherence to all DOT regulatory requirements before any employment action is taken.
Employers must maintain strict record-keeping protocols for all DOT documentation. For employers covered by the Federal Motor Carrier Safety Administration (FMCSA), the DER is also responsible for reporting specific violations to the Drug and Alcohol Clearinghouse. This includes verified positive drug test results, refusals to test, and actual knowledge violations, ensuring a centralized national repository.
Standard Timelines for Receiving DOT Results
The turnaround time for a drug test result depends heavily on the outcome of the laboratory analysis. For negative results, the MRO typically verifies and reports the finding to the DER within 24 to 48 hours of the lab receiving the specimen.
When the lab reports a non-negative result, the timeline is extended significantly due to the mandatory MRO review and employee consultation. This verification stage can take up to five business days or longer. The duration depends on the MRO’s attempts to contact the employee and the speed at which the employee provides necessary medical documentation.
Steps for Handling Delayed or Disputed Results
If a result is significantly delayed past the standard timeline, the employee should first contact the Designated Employer Representative (DER) to confirm the MRO has received the testing paperwork. Delays often stem from logistical issues, such as missing documentation on the CCF or administrative hold-ups. A simple inquiry can often prompt the tracking of the specimen or the paperwork.
When an employee disputes a verified positive result, they have a federal right to request a test of the split specimen. The original collection procedure requires the specimen to be divided into two separate bottles: the primary and the split. The employee must make this request to the MRO within 72 hours of being notified.
The MRO arranges for the split specimen to be sent to a different SAMHSA-certified laboratory for analysis. If the second laboratory fails to reconfirm the presence of the drug, the MRO cancels the verified positive result.

